DNA sequence reverse complement tool: Enter a DNA sequence and the program will give you the reverse sequence, complement sequence, or reverse and complement sequence.Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. Codon optimization tool: To access this tool, set up an IDT account.įigure 2: Addgene's Analyze Sequence tool.UniProt: A database of protein sequence and functional information, such as expression patterns, post-translational modifications and polymorphisms.ExPASy translation tool: Translates a DNA sequence using all six open reading frames.NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database.Addgene vector database: Find sequences for plasmids assembled from publications and commercially available sources, as well as those deposited with Addgene.Webcutter 2.0: For finding restriction enzyme cut sites.NEBCutter2: For finding restriction enzyme cut sites.Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates a sequence.Wayne Davis from the University of Utah.įigure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.ĭNA / protein database search and analysis tools ApE (A plasmid Editor): A free, donation-based plasmid analysis tool including editing, annotating, creating maps, and more.
#N NUCELOTIDE SEQUENCE SNAPGENE VIEWER SERIAL#
Serial Cloner: Free desktop-based software for plasmid design and mapping.Benchling: While you might think of Benchling as an electronic lab notebook, it also has a suite of molecular biology tools and can make plasmid maps.SnapGene Viewer: The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure 1).These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate any DNA sequence. To help you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology tools all in one place. We all have our favorite tools for tackling these particular tasks, but they tend to be scattered about the internet. The remaining pork belly were processed into Chashu and used as a topping for ramen.Primer design. Surprisingly I had no problem adapting to them and I realized how they are a perfect match for the pork belly Raw Chinese chives and coriander seemed intimidating to me at first but To make the nước chấm sauce,ĭissolve sucrose in fish sauce, mix with freshly squeezed lime juice and garnish with finely chopped garlicĪnd rings of bird’s eye chillies. & cucumber strands, bean sprouts, lettuce, mint, coriander, and Chinese chive). Ingredients of choice (I used a customary combination of pork belly, king prawns, rice vermicelli, carrot Gỏi cuốn (Vietnamese spring rolls) were made by rolling up dampened bánh tráng (rice paper) to wrap the Since it does not require much effort I might routinely enjoy it from now on. This is the first time I make Tteokguk and I It with spring onions and thin strips of fried egg white & yolk. The Tteokguk was made by boiling Tteok (Korean rice cakes) in Shiitake mushrooms & beef Galbi soup and topping It is a great chance for me to try making some Korean and Vietnamese festive dishes. To celebrate the lunar New Year of the Rabbit, I cooked a Szechuanese Nianyefan, featuring Hot & Spicy Diced Rabbit, Mapo Tofu and Hui Guo Rou, accompanied by Pimm’s No.1 Cup Platinum Jubilee Edition and Guo Jiao 1573.
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